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Part:BBa_K1554000:Design

Designed by: Valencia_UPV team members   Group: iGEM14_Valencia_UPV   (2014-09-26)


TA29 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was obtained by PCR amplification using Phusion polymerase and afterwards sequenced.

It was designed for GoldenBraid 2.0, a Golden Gate-based assembly system, so it has the overhangs required (GGAG and AATG). This part was sent to the Registry of Parts using RFP coding device - Golden Gate Module Flipper.

Source

Genomic DNA from Nicotiana tabacco.

References

[1] Mariani, C., De Beuckeleer, M., Truettner, J., Leemans, J., and Goldberg, R.B. (1990). lnduction of male sterility in plants by a chimaeric ribonuclease gene. Nature, 347, 737–741.

[2] Cho HJ, Kim S, Kim M, Kim BD (2001) Production of transgenic male sterile tobacco plants with the cDNA encoding a ribosome inactivating protein in Dianthus sinensis L. Mol Cells 11:326–333

[3] Sa G, Mi M, He-Chun Y, Guo-Feng L (2002) Anther-specific expression of ipt gene in transgenic tobacco and its effect on plant development. Transgenic Res 11:269–278

[4] Shaya F, Gaiduk S, Keren I, Shevtsov S, Zemah H, Belausov E, Evenor D, Reuveni M, Ostersetzer-Biran O (2012) Expression of mitochondrial gene fragments within the tapetum induce male sterility by limiting the biogenesis of the respiratory machinery in transgenic tobacco. J Integr Plant Biol 54:115–130

[5] Kriete, G., Niehaus, K., Perlick, A. M., Puhler, A., & Broer, I. (1996) Male sterility in transgenic tobacco plants induced by tapetum-specific deacetylation of the externally applied non-toxic compounds N-acetyl- L -phosphinothricin. The Plant Journal, 9, 809–818.